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+Observe that the side chain has three methylene groups, so that despite the fact that the terminal amino group might be charged below physiological situations, the side chain does have vital hydrophobic character. Incubate the membrane for 20 min and then wash with DMF to remove uncoupled amino acids. Immunofluorescent staining of Human melanoma cells, utilizing Rabbit polyclonal to acetyl Lysine (ab21623) at 1:a hundred dilution.
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